Numerous, somewhat woody stems grow upward to form a foliage mound 612 tall. An initial amount of 53 mg of soybean cell culture was grown in 0 mgl 1. Sucrose metabolizing enzymes in cell suspension cultures of. Pyridoxine, nicotinic acid and myoinositol present in the media had no adverse effects on growth individually. Using plant tissue culture techniques thesis pdf available june 2005 with 309 reads. Thymus vulgaris is an evergreen shrub growing to 0. In this sense, browning hampers the establishment of tissue culture in aloe abrie. Its regenerative capacity and response to culture conditions are. Thymus vulgaris belongs to the sixth section of thymus. Classification of this species is difficult and therefore methods of comparative enzyme study may be useful for identification elenarossello 1979.
Effect of vitamins on in vitro organogenesis of plant. Tissue culture technology is used for the production of doubled haploids. Pdf production of thymol from callus tissue of thyme. Bean, meristem, mature plant development, cytokinins, tissue culture. Agno3 is widely used in plant tissue culture as a potent. Media used in plant tissue culture are composed of seve ral com ponents. Thymus vulgaris, commonly called garden thyme or common thyme, is a generally upright, woodybased perennial which is primarily grown as a culinary herb in herb gardens. In vitro inhibition of human leukemia thp1 cells by. Tissue culture involves the use of small pieces of plant tissue explants which are cultured in a nutrient medium under sterile conditions. In vitro plant regeneration system for common bean phaseolus vulgaris. The growth kinetics of the cell suspension culture revealed a maximum cell growth exponential growth phase at day 16 and an 80% cell. Plant tissue culture broadly refers to the in vitro cultivation of plants, seeds and various parts of the plants organs, embryos, tissues, single cells, protoplasts. As sucrose concentration in the culture medium decreased, sucrose synthase activity increased in c. Regeneration of phaseolus vulgaris from epicotyls and hypocotyls.
We studied the growth and development of phaseolus vulgaris l. The cultivation process is invariably carried out in a nutrient culture medium under aseptic conditions. The protocol reported here enables propagation of p. Production of thymol from callus tissue of thyme thymus vulgaris l. The regeneration of bean plants from meristem culture zobodat.
It is in leaf all year, in flower from june to august. By plant tissue culture new plants may be raised in an artificial medium from very small parts of plants, such as, shoot tip, root tip, callus, seed, embryo, pollen grain, ovule or even a single cell, whether the cultured tissue develops into a plant or grows unorganized depends on the genetic potential of the tissue and the chemical and physical environment. After 5 days, 8 mg and 203 mg of soybean cells were produced, respectively. Tissue culture is the growth of tissues or cells in an artificial medium separate from the organism. Jalas 1972 in flora europaea, gives 66 species of thymus, divided into two subgenera, coridothymus, with only one species, and thymus with 65 species. Thymus vulgaris common thyme, garden thyme, wild thyme. This is typically facilitated via use of a liquid, semisolid, or solid. Plant cell and tissue culture phytopathology biochemicals.1478 353 556 1503 1333 457 671 782 137 13 108 268 670 941 309 1031 1500 1339 1521 560 513 235 1285 848 847 1149 970 1059 775 1539 1518 985 299 817 891 1379 862 678 1433 901 322 1154 1474 793 776 332